QuantiGene 2.0 DNA Assay - Benefits
- Detect original DNA population - using signal amplification
- No DNA purification
- No reverse transcription
- No PCR amplification
- Avoid biases and sample loss - with direct from lysate quantitation using signal amplification
- No target loss during DNA isolation
- No biases associated with enzyme inhibition or fluorescent dye incorporation during PCR amplification
- No target loss during DNA isolation
- Simplified workflow - with direct from lysate quantitation
- Lyse and go sample preparation
- ELISA like protocol - add and wash reagetns
- Lyse and go sample preparation
- Sensitive quantitation
- Sensitivity comparable to real time PCR (limit of detection)
- Precisely quantify as littel as 20% difference in copy number
- Sensitivity comparable to real time PCR (limit of detection)
- Analyze any sample, any target
- DNA copy number quantitation directly from any sample - cultured cells; whole blood; dried blood spots; fresh, frozen or formalin-fixed, paraffin-embedded (FFPE) tissues; plants; bacteria; purified DNA.
- Create probe sets against any target sequence
- Read using a standard plate luminometer
QuantiGene 2.0 - Applications
- DNA Assay - used for copy number determination in transgenic animals, plant zygocity testing.
QuantiGene 2.0 Assay Workflow
QuantiGene assays bring the power of branched DNA (bDNA) technology to basic and pre-clinical research. The bDNA assay is a sandwich nucleic acid hybridization method that uses bDNA molecules to amplify signal from captured target RNA. bDNA technology forms the basis of the FDA-approved clinical diagnostic VERSANT 3.0 assays for HIV, HCV and HBV viral load, that are offered commercially by Siemens and have been in use for over a decade. Another advantage of bDNA assays is that RNA is measured directly from the sample source, without RNA purification or enzymatic manipulation, thereby avoiding inefficiencies and variability introduced by or errors inherent to these processes.
Assay Specifications
|
a Defined as signal greater than background plus three standard deviations of the background.
b Defined as the signal just above the lowest signal that obtains an 80-120% spike recovery.
c Defined as the assay window that consistently achieves a 80-120% accuracy of fold change.
d Contact Affymetrix for information on a 384-well version.
e Batch or full automation using standard automation equipment. Contact Technical Support for protocol details and assistance in setting up your automation system.
