Affymetrix | Panomics Solutions
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Quantitative Biology Delivered.

QuantiGene 2.0 - Benefits

  • Detect original RNA or DNA population - using signal amplification
    • No RNA or DNA purification
    • No reverse transcription
    • No PCR amplification
  • Avoid biases and sample loss - with direct from lysate quantitation using signal amplification
    • No target loss during RNA/DNA isolation
    • No biases associated with cDNA synthesis
    • No biases associated with enzyme inhibition or fluorescent dye incorporation during PCR amplification
  • Simplified workflow - with direct from lysate quantitation
    • Lyse and go sample preparation
    • ELISA like protocol - add and wash reagetns
  • Sensitive quantitation
    • Sensitivity comparable to real time PCR (limit of detection)
    • Precisely quantify as littel as 10% difference in gene expression
  • Analyze any sample, any target
    • RNA quantitation directly from any sample - cultured cells; whole blood; dried blood spots; fresh, frozen or formalin-fixed, paraffin-embedded (FFPE) tissues; plants; bacteria; purified RNA and DNA. 
    • Create probe sets against any target sequence
  • Read using a standard plate luminometer

 

QuantiGene 2.0 - Applications

  • RNA Assay - widely used in biomarker discovery, secondary screening, microarray validation, quantification of RNAi knockdowns and predictive toxicology
  • DNA Assay - used for copy number determination in transgenic animals, plant zygocity testing

 

QuantiGene 2.0 - Assay Workflow

QuantiGene assays bring the power of branched DNA (bDNA) technology to basic and pre-clinical research. The bDNA assay is a sandwich nucleic acid hybridization method that uses bDNA molecules to amplify signal from captured target RNA. bDNA technology forms the basis of the FDA-approved clinical diagnostic VERSANT 3.0 assays for HIV, HCV and HBV viral load, that are offered commercially by Siemens and have been in use for over a decade. Another advantage of bDNA assays is that RNA is measured directly from the sample source, without RNA purification or enzymatic manipulation, thereby avoiding inefficiencies and variability introduced by or errors inherent to these processes.

QuantiGene 2.0 Assay Specifications


  Limit of Detection   ≤ 200 transcripts/assay well
  Limit of Quantitation   ≤ 500 transcripts/assay well
  Linear Dynamic Range   ≥ 3.5 logs
  Assay CV   ≤ 10% intra-assay; ≤ 15% inter-assay
  Compatible Sample Types   Cultured cells, bacteria, whole blood,
  PAXgene blood or dried blood spots,
  fresh/frozen tissues (animal or plant),
  FFPE samples, purified RNA
  Assay Format   96-well plate
  Targets/well   1