- Affymetrix offers a 41 Plex Panel and a second 44 plex panel for a total of 85 Luminex assays
- Profile the DNA binding activity of up to 44 different transcription factors per Panel (TFs) in a single well
- Mix and Match to create your own plex set from 3-44 Plex
- Quantitate TF binding activity from whole cell lysates or nuclear extracts
- Complete assay in 5 hours
Transcription factors (TFs), which play crucial roles in the regulation of gene expression in the human genome, are highly regulated by a variety of mechanisms. A single extracellular stimulus can trigger multiple signaling pathways, and these in turn can activate multiple TFs to mediate the inducible expression of target genes. Alterations in the activities of TFs are often associated with human diseases, such as altered activating factor 1, estrogen receptor, and p53 function in cancer, nuclear factor kB in inflammatory diseases, and peroxisome proliferator-activated receptor gamma in obesity. A systematic assay for profiling the activation of TFs will aid in elucidating the mechanisms of TF activation, reveal altered TFs associated with human diseases, and aid in developing assays for drug discovery. We have developed a 40-plex fluorescent microsphere-based TF activation assay system with a 96-well plate format. The assay system enables high-throughput profiling of the DNA binding activity of TFs in multiple samples with high sensitivity.
Procarta Transcription Factor assays combine two technologies: Affymetrix' proprietary detection probe separation method and xMAP® (multi-analyte profiling beads). Together, these two technologies enable the quantitative measurement of DNA binding activity of up to 40 transcription factors (human, mouse or rat) from as little as 500 ng of protein from a whole cell lysate or 250 ng of protein from a nuclear extract. The xMAP system, developed by Luminex Corp., combines flow cytometry, fluorescent-dyed microspheres (beads), lasers and digital signal processing to effectively allow multiplexing of up to 44 unique assays within a single sample.
