Fluorescence RNA In Situ Hybridization (RNA FISH) - Overview
 |
Fluorescence based in situ hybridization (FISH) techniques are used to visualize DNA or localized RNAs within cells. However the fluorescence in situ hybridization of RNA has always been limited by low sensitivity, complicated workflow and the inability to perform multiplex analysis. QuantiGene ViewRNA HC Screening Assay is a novel non-radioactive RNA in situ hybridization solution that offers single-molecule RNA sensitivity and the ability to multiplex up to 4 RNA targets in adherent or suspension cells. The assay is optimized for automation and can be performed in 96- or 384-well plates. |
Unique Benefits
- Directly visualize RNA in adherent or suspension cells
- Sensitivity of one copy/cell at single cell resolution
- Analyze one to four mRNA targets per cell
- High-throughput and high-content compatible
- Compatible with standard fluorescence microscopes or high content imagers
Applications
- siRNA knockdown studies
- Stem cells and developmental biology
- Biomarker validation
- Analysis of transcriptional heterogeneity
- Reporter gene assays
- Time course studies
- Viral gene trafficking
- A companion to immunofluorescence (IF) or DNA FISH assays
Assay Performance Highlights
QuantiGene® ViewRNA HC Screening Assay |
|
| Sample Type | Cultured cells (adherent and suspension) |
| Species | Any |
| Limit of Detection (LOD) | 1 mRNA molecule/cell |
| Plex Level | 1 to 4-plex |
| Assay Format | 96- or 384-well plates |
| Detection | Fluorescent |
| Instrumentation | Imaging system of epi-fluorescence microscope |
